Anti-phospho-FOXO4 (pSer197) antibody produced in rabbit
SIGMA/SAB4300113 - affinity isolated antibody
Synonym: Anti-AFX antibody produced in rabbit; Anti-AFX1 antibody produced in rabbit; Anti-MGC120490 antibody produced in rabbit; Anti-MLLT7 antibody produced in rabbit; Anti-forkhead box O4 antibody produced in rabbit
MDL Number: MFCD03791410
Product Type: Chemical
Immunohistochemistry Anti-phospho-FOXO4 (pSer197): SAB4300113: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-FOXO4 (pSer197) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-FOXO4 (pSer197): SAB4300113: Staining of methanol-fixed MCF7 cells using Anti-phospho-FOXO4 (pSer197) antibody (Red).
Immunoblotting Anti-phospho-FOXO4 (pSer197): SAB4300113: Analysis of extracts from 293 cells untreated or treated with serum using Anti-phospho-FOXO4 (pSer197) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (A-A-SP-M-D) |
| isotype | IgG |
| mol wt | ~65 kDa |
| NCBI accession no. | NP_001164402.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer197) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P98177 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D), according to the protein FOXO4. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |