Anti-phospho-CFL1 (pSer3) antibody produced in rabbit
SIGMA/SAB4300115 - affinity isolated antibody
Synonym: Anti-CFL antibody produced in rabbit; Anti-cofilin 1 (non-muscle) antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-phospho-CFL1 (pSer3): SAB4300115: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-CFL1 (pSer3) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-CFL1 (pSer3): SAB4300115: Staining of methanol-fixed HeLa cells using Anti-phospho-CFL1 (pSer3) antibody (Red).
Immunoblotting Anti-phospho-CFL1 (pSer3): SAB4300115: Analysis of extracts from COLO205 cells using Anti-phospho-CFL1 (pSer3) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (M-A-SP-G-V) |
| isotype | IgG |
| mol wt | ~19 kDa |
| NCBI accession no. | NP_005498.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer3) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P23528 |
| Application: | Anti-phospho-CFL1 (pSer3) antibody produced in rabbit is used in Western Blotting. |
| Biochem/physiol Actions: | Cofilin-l (CFL1) plays a vital role in the regulation of actin cytoskeleton reorganization. It facilitates cytokinesis, cell motility and morphogenesis. CFL1 is also implicated in cancer cell migration and invasion in several types of tumor tissues. Elevated expression of the protein is associated with the development of esophageal squamous cell carcinoma (ESCC). In addition, its abnormal expression leads to vulvar cancer and thus, it can be considered as a potential therapeutic target for the same. Activated CFL1 helps in preventing cytoskeletal-associated defects in axonal development stimulated by synucleins (Syns). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. |
| General description: | The cofilin 1 (CFL1) gene is mapped to human chromosome 11q13.1. It has four exons. The CFL1 gene codes for a ubiquitously expressed, 18.5 kDa phosphoprotein. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V), according to the protein CFL1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |