Anti-phospho-CHEK2 (pSer516) antibody produced in rabbit
SIGMA/SAB4300121 - affinity isolated antibody
Synonym: Anti-CDS1 antibody produced in rabbit; Anti-CHK2 antibody produced in rabbit; Anti-CHK2 checkpoint homolog (S. pombe) antibody produced in rabbit; Anti-HuCds1 antibody produced in rabbit; Anti-LFS2 antibody produced in rabbit
Product Type: Chemical
Immunoblotting Anti-phospho-CHEK2 (pSer516): SAB4300121: Analysis of extracts from A549 cells untreated or treated with Anisomycin using Anti-phospho-CHEK2 (pSer516) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (Q-P-SP-T-S) |
| isotype | IgG |
| mol wt | ~62 kDa |
| NCBI accession no. | NP_001005735.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer516) |
| technique(s) | western blot: 1:500-1:1000 |
| UniProt accession no. | O96017 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 516 (Q-P-S(p)-T-S), according to the protein CHEK2. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |