Anti-phospho-NFKBIA (pSer32/pSer36) antibody produced in rabbit
SIGMA/SAB4300125 - affinity isolated antibody
Synonym: Anti-IKBA antibody produced in rabbit; Anti-MAD-3 antibody produced in rabbit; Anti-NFKBI antibody produced in rabbit; Anti-nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha antibody produced in rabbit
MDL Number: MFCD01633603
Product Type: Chemical
Immunohistochemistry Anti-phospho-NFKBIA (pSer32/pSer36): SAB4300125: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-NFKBIA (pSer32/pSer36) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-NFKBIA (pSer32/pSer36): SAB4300125: Staining of methanol-fixed HeLa cells using Anti-phospho-NFKBIA (pSer32/pSer36) antibody (Red).
Immunoblotting Anti-phospho-NFKBIA (pSer32/pSer36): SAB4300125: Analysis of extracts from HeLa cells untreated or treated with TNF using Anti-phospho-NFKBIA (pSer32/pSer36) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (H-D-SP-G-L-D-SP-M-K) |
| isotype | IgG |
| mol wt | ~39 kDa |
| NCBI accession no. | NP_065390.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer32/pSer36) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P25963 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p) -M-K), according to the protein NFKBIA. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |