Anti-phospho-STAT1 (pSer727) antibody produced in rabbit
SIGMA/SAB4300133 - affinity isolated antibody
Synonym: Anti-DKFZp686B04100 antibody produced in rabbit; Anti-ISGF-3 antibody produced in rabbit; Anti-STAT91 antibody produced in rabbit; Anti-signal transducer and activator of transcription 1, 91kDa antibody produced in rabbit
MDL Number: MFCD01633987
Product Type: Chemical
Immunohistochemistry Anti-phospho-STAT1 (pSer727): SAB4300133: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-STAT1 (pSer727) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-STAT1 (pSer727): SAB4300133: Staining of methanol-fixed HeLa cells using Anti-phospho-STAT1 (pSer727) antibody (Red).
Immunoblotting Anti-phospho-STAT1 (pSer727): SAB4300133: Analysis of extracts from HeLa cells using Anti-phospho-STAT1 (pSer727) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (P-M-SP-P-E) |
| isotype | IgG |
| mol wt | ~91 kDa |
| NCBI accession no. | NP_009330.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer727) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P42224 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 727 (P-M-S(p)-P-E), according to the protein STAT1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |