Anti-phospho-YWHAZ (pSer58) antibody produced in rabbit
SIGMA/SAB4300147 - affinity isolated antibody
Synonym: Anti-KCIP-1 antibody produced in rabbit; Anti-MGC111427 antibody produced in rabbit; Anti-MGC126532 antibody produced in rabbit; Anti-MGC138156 antibody produced in rabbit; Anti-tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, zeta polypeptide antibody produced in rabbit
MDL Number: MFCD01324139
Product Type: Chemical
Immunohistochemistry Anti-phospho-YWHAZ (pSer58): SAB4300147: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-YWHAZ (pSer58) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-YWHAZ (pSer58): SAB4300147: Staining of methanol-fixed HeLa cells using Anti-phospho-YWHAZ (pSer58) antibody (Red).
Immunoblotting Anti-phospho-YWHAZ (pSer58): SAB4300147: Analysis of extracts from 3T3 cells untreated (Lane 1) or treated with TNF-α (Lane 2) using Anti-phospho-YWHAZ (pSer58) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (R-S-SP-W-R) |
| isotype | IgG |
| mol wt | ~28 kDa |
| NCBI accession no. | NP_001129171.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer58) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P63104 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R), according to the protein YWHAZ. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |