Anti-phospho-ADD1 (pSer726) antibody produced in rabbit
SIGMA/SAB4300148 - affinity isolated antibody
Synonym: Anti-ADDA antibody produced in rabbit; Anti-MGC3339 antibody produced in rabbit; Anti-MGC44427 antibody produced in rabbit; Anti-adducin 1 (alpha) antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-phospho-ADD1 (pSer726): SAB4300148: Analysis of paraffin-embedded human lung carcinoma tissue using Anti-phospho-ADD1 (pSer726) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-ADD1 (pSer726): SAB4300148: Staining of methanol-fixed HeLa cells using Anti-phospho-ADD1 (pSer726) antibody (Red).
Immunoblotting Anti-phospho-ADD1 (pSer726): SAB4300148: Analysis of extracts from HeLa cells using Anti-phospho-ADD1 (pSer726) antibody and the same antibody preincubated with blocking peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (T-P-SP-F-L) |
| isotype | IgG |
| mol wt | ~130 kDa |
| NCBI accession no. | NP_001110.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer726) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P35611 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 726 (T-P-S(p)-F-L), according to the protein ADD1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |