Anti-phospho-MAPK3 (pTyr204) antibody produced in rabbit
SIGMA/SAB4300195 - affinity isolated antibody
Synonym: Anti-ERK1 antibody produced in rabbit; Anti-HS44KDAP antibody produced in rabbit; Anti-HUMKER1A antibody produced in rabbit; Anti-MGC20180 antibody produced in rabbit; Anti-
MDL Number: MFCD02179320
Product Type: Chemical
Immunohistochemistry Anti-phospho-MAPK3 (pTyr204): SAB4300195: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-MAPK3 (pTyr204) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-MAPK3 (pTyr204): SAB4300195: Staining of methanol-fixed HeLa cells showing centrosome and nuclear staining using Anti-phospho-MAPK3 (pTyr204) antibody.
Immunoblotting Anti-phospho-MAPK3 (pTyr204): SAB4300195: Analysis of extracts from 293 cells untreated or treated with UV using Anti-phospho-MAPK3 (pTyr204) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (T-E-YP-V-A) |
| isotype | IgG |
| mol wt | ~42 kDa |
| ~44 kDa | |
| NCBI accession no. | NP_001035145.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr204) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P27361 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. |
| Immunogen: | Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A), according to the protein MAPK3. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |