Anti-phospho-MAPK14 (pThr180) antibody produced in rabbit
SIGMA/SAB4300200 - affinity isolated antibody
Synonym: Anti-CSBP1 antibody produced in rabbit; Anti-CSBP2 antibody produced in rabbit; Anti-CSPB1 antibody produced in rabbit; Anti-EXIP antibody produced in rabbit; Anti-
Product Type: Chemical
Immunohistochemistry Anti-phospho-MAPK14 (pThr180): SAB4300200: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-MAPK14 (pThr180) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunoblotting Anti-phospho-MAPK14 (pThr180): SAB4300200: Analysis of extracts from HT29 cells untreated or treated with UV using Anti-phospho-MAPK14 (pThr180) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (E-M-TP-G-Y) |
| isotype | IgG |
| mol wt | ~43 kDa |
| NCBI accession no. | NP_001306.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr180) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q16539 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. |
| Immunogen: | Peptide sequence around phosphorylation site of threonine 180 (E-M-T(p)-G-Y ), according to the protein MAPK14. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |