Anti-phospho-H2AFX (pSer139) antibody produced in rabbit
SIGMA/SAB4300213 - affinity isolated antibody
Synonym: Anti-H2A histone family, member X antibody produced in rabbit; Anti-H2A.X antibody produced in rabbit; Anti-H2A/X antibody produced in rabbit; Anti-H2AX antibody produced in rabbit
MDL Number: MFCD07370338
Product Type: Chemical
Immunofluorescence Anti-phospho-H2AFX (pSer139): SAB4300213: Staining of methanol-fixed HeLa cells using Anti-phospho-H2AFX (pSer139) antibody (Red).
Immunoblotting Anti-phospho-H2AFX (pSer139): SAB4300213: Analysis of extracts from HT29 cells untreated (Lane 1) or treated with UV (Lane 2) using Anti-phospho-H2AFX (pSer139) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (Q-A-SP-Q-E) |
| isotype | IgG |
| mol wt | ~15 kDa |
| NCBI accession no. | NP_002096.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer139) |
| technique(s) | indirect immunofluorescence: 1:100-1:200 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P16104 |
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| General description: | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E), according to the protein H2AFX. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |