Anti-phospho-EIF2AK2 (pThr446) antibody produced in rabbit
SIGMA/SAB4300222 - affinity isolated antibody
Synonym: Anti-EIF2AK1 antibody produced in rabbit; Anti-MGC126524 antibody produced in rabbit; Anti-PKR antibody produced in rabbit; Anti-PRKR antibody produced in rabbit; Anti-eukaryotic translation initiation factor 2-alpha kinase 2 antibody produced in rabbit
MDL Number: MFCD03455796
Product Type: Chemical
Immunohistochemistry Anti-phospho-EIF2AK2 (pThr446): SAB4300222: Analysis of paraffin-embedded human colon carcinoma tissue using Anti-phospho-EIF2AK2 (pThr446) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-EIF2AK2 (pThr446): SAB4300222: Staining of methanol-fixed HeLa cells using Anti-phospho-EIF2AK2 (pThr446) antibody (Red).
Immunoblotting Anti-phospho-EIF2AK2 (pThr446): SAB4300222: Analysis of extracts from K562 cells using Anti-phospho-EIF2AK2 (pThr446) antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (K-R-TP-R-S) |
| isotype | IgG |
| mol wt | ~74 kDa |
| NCBI accession no. | NP_001129123.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr446) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P19525 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. |
| Immunogen: | Peptide sequence around phosphorylation site of threonine 446 (K-R-T(p)-R-S), according to the protein EIF2AK2. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |