Anti-phospho-SHC1 (pTyr349) antibody produced in rabbit
SIGMA/SAB4300245 - affinity isolated antibody
Synonym: Anti-FLJ26504 antibody produced in rabbit; Anti-SHC (Src homology 2 domain containing) transforming protein 1 antibody produced in rabbit; Anti-SHC antibody produced in rabbit; Anti-SHCA antibody produced in rabbit
MDL Number: MFCD01634176
Product Type: Chemical
Immunofluorescence Anti-phospho-SHC1 (pTyr349): SAB4300245: Staining of methanol-fixed HeLa cells using Anti-phospho-SHC1 (pTyr349) antibody (Red).
Immunoblotting Anti-phospho-SHC1 (pTyr349): SAB4300245: Analysis of extracts from HepG2 cells untreated or treated with EGF using Anti-phospho-SHC1 (pTyr349) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (H-Q-YP-Y-N) |
| isotype | IgG |
| mol wt | ~46 kDa |
| ~52 kDa | |
| NCBI accession no. | NP_001123512.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr349) |
| technique(s) | indirect immunofluorescence: 1:100-1:200 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P29353 |
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| General description: | Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span. |
| Immunogen: | Peptide sequence around phosphorylation site of tyrosine 349 (H-Q-Y(p)-Y-N), according to the protein SHC1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |