Anti-phospho-SMAD3 (pSer425) antibody produced in rabbit
SIGMA/SAB4300253 - affinity isolated antibody
Synonym: Anti-DKFZp586N0721 antibody produced in rabbit; Anti-DKFZp686J10186 antibody produced in rabbit; Anti-HSPC193 antibody produced in rabbit; Anti-HsT17436 antibody produced in rabbit; Anti-SMAD family member 3 antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-phospho-SMAD3 (pSer425): SAB4300253: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-SMAD3 (pSer425) antibody (left) or the same antibody preincubated with blocking peptide (right).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (C-S-S-V-SP) |
| isotype | IgG |
| mol wt | ~52 kDa |
| NCBI accession no. | NP_001138574.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer425) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| UniProt accession no. | P84022 |
| Application: | Anti-phospho-SMAD3 (pSer425) antibody produced in rabbit has been used in immunoblotting. |
| Biochem/physiol Actions: | Smad3 (mothers against decapentaplegic homolog 3) is associated with TGF-β (transforming growth factor β) signaling pathway, a cellular process regulating the homeostasis, development, and repair of cartilage. It is known to induce extracellular matrix production. Smad proteins are responsible for the transduction of signals from cell surface to the nucleus. Phosphorylation of Smad3 controls tumor progression, inflammation, fibrosis, obesity and diabetes. Smad3 expression might be associated with the pathogenesis of osteoarthritis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. |
| General description: | The SMAD3 (mothers against decapentaplegic homolog 3) gene is mapped to human chromosome 15q22.33. Smad3 protein identifies tandem repeats of the palindromic sequence GTCTAGAC, which is part of TGF-β (Transforming growth factor β) signaling promoter region. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)), according to the protein SMAD3. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |