Anti-phospho-AKT1/AKT2/AKT3 (pTyr315/pTyr316/pTyr312) antibody produced in rabbit
SIGMA/SAB4300259 - affinity isolated antibody
Synonym: Anti-AKT antibody produced in rabbit; Anti-DKFZp434N0250 antibody produced in rabbit; Anti-PKBB antibody produced in rabbit; Anti-v-akt murine thymoma viral oncogene homolog 1 / 2 / 3 (protein kinase B, gamma) antibody produced in rabbit
MDL Number: MFCD03096597
Product Type: Chemical
Immunohistochemistry Anti-phospho-AKT1/AKT2/AKT3 (pTyr315/pTyr316/pTyr312): SAB4300259: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-AKT1/AKT2/AKT3 (pTyr315/pTyr316/pTyr312) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-AKT1/AKT2/AKT3 (pTyr315/pTyr316/pTyr312): SAB4300259: Staining of methanol-fixed HeLa cells using Anti-phospho-AKT1/AKT2/AKT3 (pTyr315/pTyr316/pTyr312) antibody (Red).
Immunoblotting Anti-phospho-AKT1/AKT2/AKT3 (pTyr315/pTyr316/pTyr312): SAB4300259: Analysis of extracts from HepG2 cells untreated or treated with EGF using Anti-phospho-AKT1/AKT2/AKT3 (pTyr315/pTyr316/pTyr312) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (P-E-YP-L-A) |
| isotype | IgG |
| mol wt | ~60 kDa |
| NCBI accession no. | NP_001014431.1   |
| NP_001617.1 |
|
| NP_005456.1 |
|
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr315/pTyr316/pTyr312) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P31751 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. /IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. |
| Immunogen: | Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y(p)-L-A), according to the proteins AKT1/AKT2/AKT3. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |