Anti-phospho-SYK (pTyr323) antibody produced in rabbit
SIGMA/SAB4300282 - affinity isolated antibody
Synonym: Anti-DKFZp313N1010 antibody produced in rabbit; Anti-FLJ25043 antibody produced in rabbit; Anti-FLJ37489 antibody produced in rabbit; Anti-spleen tyrosine kinase antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-phospho-SYK (pTyr323): SAB4300282: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-SYK (pTyr323) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-SYK (pTyr323): SAB4300282: Staining of methanol-fixed HeLa cells using Anti-phospho-SYK (pTyr323) antibody (Red).
Immunoblotting Anti-phospho-SYK (pTyr323): SAB4300282: Analysis of extracts from JK cells untreated or treated with H2O2 for the indicated times, using Anti-phospho-SYK (pTyr323) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (N-P-YP-E-P) |
| isotype | IgG |
| mol wt | ~72 kDa |
| NCBI accession no. | NP_001128524.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pTyr323) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P43405 |
| Biochem/physiol Actions: | Spleen tyrosine kinase (SYK) is a tumor suppressor gene that helps to prevent the development and metastasis of breast cancer cells. It participates in B-cell receptor signaling. The protein helps to phosphorylate the α-tubulin subunits of microtubules. SYK aids in the coupling of receptors for antigen-antibody complexes. It also modulates cell proliferation and differentiation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Spleen tyrosine kinase (SYK) is a member of the non-receptor tyrosine kinase family. It is usually expressed in epithelial breast tissues and hematopoietic cells. The gene encoding it is localized on human chromosome 9q22. |
| Immunogen: | Peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y(p)-E- P), according to the protein SYK. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |