Anti-phospho-MDM2 (pSer166) antibody produced in rabbit
SIGMA/SAB4300284 - affinity isolated antibody
Synonym: Anti-HDMX antibody produced in rabbit; Anti-MGC71221 antibody produced in rabbit; Anti-Mdm2 p53 binding protein homolog (mouse) antibody produced in rabbit; Anti-hdm2 antibody produced in rabbit
MDL Number: MFCD00282354
Product Type: Chemical
Immunohistochemistry Anti-phospho-MDM2 (pSer166): SAB4300284: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-MDM2 (pSer166) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-phospho-MDM2 (pSer166): SAB4300284: Staining of methanol-fixed HeLa cells using Anti-phospho-MDM2 (pSer166) antibody (Red).
Immunoblotting Anti-phospho-MDM2 (pSer166): SAB4300284: Analysis of extracts from 293 cells untreated or treated with Hydroxyurea using Anti-phospho-MDM2 (pSer166) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (A-I-SP-E-T) |
| isotype | IgG |
| mol wt | ~90 kDa |
| NCBI accession no. | NP_002383.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer166) |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q00987 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. |
| Immunogen: | Peptide sequence around phosphorylation site of serine 166 (A-I-S(p)-E-T), according to the protein MDM2. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |