Anti-EGFR (Ab-1070) antibody produced in rabbit
SIGMA/SAB4300352 - affinity isolated antibody
Synonym: Anti-ERBB antibody produced in rabbit; Anti-ERBB1 antibody produced in rabbit; Anti-HER1 antibody produced in rabbit; Anti-PIG61 antibody produced in rabbit; Anti-epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-EGFR (Ab-1070): SAB4300352: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-EGFR (Ab-1070) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-EGFR (Ab-1070): SAB4300352: Staining of methanol-fixed MCF cells using Anti-EGFR (Ab-1070) antibody (Red).
Immunoblotting Anti-EGFR (Ab-1070): SAB4300352: Analysis of extracts from HUVEC and MDA cells using Anti-EGFR (Ab-1070) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (R-Y-S-S-D) |
| isotype | IgG |
| mol wt | ~175 kDa |
| NCBI accession no. | NP_005219.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P00533 |
| Application: | Anti-EGFR (Ab-1070) antibody has been used in immunohistochemistry. |
| Biochem/physiol Actions: | EGFR (epidermal growth factor receptor) plays an important role in ligand binding, dimerization and autophosphorylation. It also plays an important role in several procarcinogenic cancers like gastric cancer. It controls various functions of epithelial cell. It modulates the actions in bacterial infection and is associated with the initiation of macrophage. |
| Biochem/physiol Actions: | Upon activation by ligands, epidermal growth factor receptor (EGFR, ErbB1) initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | EGFR (epidermal growth factor receptor) belongs to the ErbB family of receptor tyrosine kinases. It is also known as ErbB1/HER1 (human epidermal growth factor receptor). It is located on chromosome 7p11.2. It has a large extracellular ligand-binding domain, a single transmembrane segment, an intracellular juxtamembrane segment, a tyrosine kinase domain and a carboxy-terminal tail. |
| General description: | Epidermal growth factor receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. EGFR can be activated by binding EGF or at least six other structurally related protein ligands, including transforming growth factor α (TGFα), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), amphiregulin, epiregulin and epigen. The gene encoding it is localized on human chromosome 7p11.2. |
| General description: | Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. |
| Immunogen: | Peptide sequence around aa. 1068-1072 (R-Y-S-S-D), according to the protein EGFR. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |