Anti-CHUK (Ab-23) antibody produced in rabbit
SIGMA/SAB4300396 - affinity isolated antibody
Synonym: Anti-IKBKA antibody produced in rabbit; Anti-IKK-alpha antibody produced in rabbit; Anti-IKK1 antibody produced in rabbit; Anti-IKKA antibody produced in rabbit; Anti-conserved helix-loop-helix ubiquitous kinase antibody produced in rabbit
MDL Number: MFCD02096685
Product Type: Chemical
Immunohistochemistry Anti-CHUK (Ab-23): SAB4300396: Analysis of paraffin-embedded human colon carcinoma tissue using Anti-CHUK (Ab-23) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunoblotting Anti-CHUK (Ab-23): SAB4300396: Analysis of extracts from HeLa, 293 and 3T3 cells using Anti-CHUK (Ab-23) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (L-G-T-G-G) |
| isotype | IgG |
| mol wt | ~85 kDa |
| NCBI accession no. | NP_001269.3   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | O15111 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. |
| Immunogen: | Peptide sequence around aa. 21-25 (L-G-T-G-G), according to the protein CHUK. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |