Anti-CTNNB1 (Ab-33) antibody produced in rabbit
SIGMA/SAB4300470 - affinity isolated antibody
Synonym: Anti-CTNNB antibody produced in rabbit; Anti-DKFZp686D02253 antibody produced in rabbit; Anti-FLJ25606 antibody produced in rabbit; Anti-FLJ37923 antibody produced in rabbit; Anti-catenin (cadherin-associated protein), beta 1, 88kDa antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-CTNNB1 (Ab-33): SAB4300470: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-CTNNB1 (Ab-33) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-CTNNB1 (Ab-33): SAB4300470: Staining of methanol-fixed HeLa cells using Anti-CTNNB1 (Ab-33) antibody (Red).
Immunoblotting Anti-CTNNB1 (Ab-33): SAB4300470: Analysis of extracts from HeLa and HT29 cells using Anti-CTNNB1 (Ab-33) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (L-D-S-G-I) |
| isotype | IgG |
| mol wt | ~92 kDa |
| NCBI accession no. | NP_001091679.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P35222 |
| Biochem/physiol Actions: | Catenin beta-1 (CTNNB1) is a signalling molecule in the nucleus and anchors actin cytoskeleton to cadherin-based adherens junctions. Phosphorylation causes dissociation of β-catenin from E-cadherin and degradation by proteasome pathway. β-catenin is a key molecule in wingless/integrated (wnt) signalling. β-catenin forms a complex with kindlin 2 and T-cell factor 4 (TCF4) to regulate wnt target gene expression. Mutations in adenomatous polyposis coli (APC) and CTNNB1 gene affects β-catenin intracellular localization and stability, contributing to aberrant expression of wnt/β-catenin in malignant melanoma. β-catenin is a major oncoprotein, which downregulates death associated protein kinase 2 (Dapk2) and inhibits anoikis of cancer cells. β-catenin expression is suppressed and its downstream gene transcription function is regulated by a tumor suppressor protein fragile histidine triad (Fhit). High expression of mucin 1 (MUC1), translocates β-catenin out of the nucleus and inhibits β-catenin regulated cell proliferation. Somatic mutations of CTNNB1 in glycogen synthase kinase 3β−casein kinase 1 (GSK-3β−CK1) phosphorylation site results in loss of phosphorylation of β-catenin. This causes aberrant wnt-signalling and leads to adrenocortical adenomas and adrenocortical carcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| General description: | Catenin beta-1 (CTNNB1) encoding β-catenin, belongs to armadillo-repeat family. In human chromosome, the gene CTNNB1 is localized on 3p22.1. |
| General description: | Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway. |
| Immunogen: | Peptide sequence around aa. 31-35 (L-D-S-G-I), according to the protein CTNNB1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |