Anti-EIF4EBP1 (Ab-45) antibody produced in rabbit
SIGMA/SAB4300475 - affinity isolated antibody
Synonym: Anti-4E-BP1 antibody produced in rabbit; Anti-4EBP1 antibody produced in rabbit; Anti-BP-1 antibody produced in rabbit; Anti-MGC4316 antibody produced in rabbit; Anti-eukaryotic translation initiation factor 4E binding protein 1 antibody produced in rabbit
MDL Number: MFCD04040379
Product Type: Chemical
Immunohistochemistry Anti-EIF4EBP1 (Ab-45): SAB4300475: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-EIF4EBP1 (Ab-45) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-EIF4EBP1 (Ab-45): SAB4300475: Staining of methanol-fixed HeLa cells using Anti-EIF4EBP1 (Ab-45) antibody (Red).
Immunoblotting Anti-EIF4EBP1 (Ab-45): SAB4300475: Analysis of extracts from 293 and HepG2 cells using Anti-EIF4EBP1 (Ab-45) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (S-T-T-P-G) |
| isotype | IgG |
| mol wt | ~18 kDa |
| NCBI accession no. | NP_004086.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q13541 |
| Biochem/physiol Actions: | The gene EIF4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) encodes a translation repressor that interacts with eukaryotic translation initiation factor 4E (eIF4E). eIF4E is a multisubunit complex that recruits 40S ribosomal subunits to the 5’ end of mRNA. The interaction of the encoded binding protein with this complex inhibits translation. The phosphorylation of eIF4Ebp1 in response to stimuli such as, UV irradiation and insulin signaling, results in dissociation of this factor from the eIF4E complex, leading to initiation of translation of mRNA. The encoded protein participates in cell proliferation, apoptosis, invasion, and metastasis. It functions as an effector molecule in mTOR (mammalian target of rapamycin complex 1) signaling pathway that regulates protein synthesis. It is found to be overexpressed in hepatocellular carcinoma tissues and serves as a potential prognostic and therapeutic target. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | 4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. |
| General description: | The gene EIF4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is mapped to human chromosome 8p12. |
| Immunogen: | Peptide sequence around aa. 43-47 (S-T-T-P-G), according to the protein EIF4EBP1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |