Anti-EIF2S1 (Ab-51) antibody produced in rabbit
SIGMA/SAB4300523 - affinity isolated antibody
Synonym: Anti-EIF-2 antibody produced in rabbit; Anti-EIF-2A antibody produced in rabbit; Anti-EIF-2alpha antibody produced in rabbit; Anti-EIF2 antibody produced in rabbit; Anti-eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-EIF2S1 (Ab-51): SAB4300523: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-EIF2S1 (Ab-51) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-EIF2S1 (Ab-51): SAB4300523: Staining of methanol-fixed HeLa cells using Anti-EIF2S1 (Ab-51) antibody (Red).
Immunoblotting Anti-EIF2S1 (Ab-51): SAB4300523: Analysis of extracts from 293 cells using Anti-EIF2S1 (Ab-51) antibody and the same antibody preincubated with blocking peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (E-L-S-R-R) |
| isotype | IgG |
| mol wt | ~38 kDa |
| NCBI accession no. | NP_004085.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P05198 |
| Biochem/physiol Actions: | EIF2S1 (eukaryotic translation initiation factor 2 subunit α) serves as an important enzyme in protein elongation during translation. EIF2S1 catalyses the translocation of peptidyl-tRNA from A site to P site in the ribosome. Phosphorylation by EF2 kinase, inactivates EIF2S1 followed by inhibition of protein synthesis. Upregulation of EIF2S1 gene is observed in malignant tumors, such as gastrointestinal cancer, lung adenocarcinoma, ovary cancer, soft-tissue sarcomas and hepatocellular carcinoma. The GTP-bound eIF2S1 protein is regarded as the active form and recruits initiator methionyl tRNA to 40S ribosomal subunit, one of the key step in translation initiation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
| General description: | The eukaryotic translation initiation factor 2 subunit α (EIF2S1) gene codes for a heterotrimeric GTPase which is localized to the cytoplasm. The EIF2S1 gene is mapped to human chromosome 14q23.3. |
| Immunogen: | Peptide sequence around aa. 49-53 (E-L-S-R-R), according to the protein EIF2S1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |