Anti-SMAD3 (Ab-208) antibody produced in rabbit
SIGMA/SAB4300564 - affinity isolated antibody
Synonym: Anti-DKFZp586N0721 antibody produced in rabbit; Anti-DKFZp686J10186 antibody produced in rabbit; Anti-HSPC193 antibody produced in rabbit; Anti-HsT17436 antibody produced in rabbit; Anti-SMAD family member 3 antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-SMAD3 (Ab-208): SAB4300564: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-SMAD3 (Ab-208) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-SMAD3 (Ab-208): SAB4300564: Staining of methanol-fixed HeLa cells using Anti-SMAD3 (Ab-208) antibody (Red).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (N-L-S-P-N) |
| isotype | IgG |
| mol wt | ~52 kDa |
| NCBI accession no. | NP_001138574.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| UniProt accession no. | P84022 |
| Biochem/physiol Actions: | SMAD3 (SMAD family member 3) plays a major role in the intracellular signaling of transforming growth factorβ(TGFβ). Reducing the phosphorylation level of Smad3 helps autophagy to control endothelial-mesenchymal transition. In arterial smooth muscle cells, suppression of SMAD3 results in elevating cell viability. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | SMAD3 (SMAD family member 3) is located on chromosome 15q22. |
| General description: | Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. |
| Immunogen: | Peptide sequence around aa. 206-210 (N-L-S-P-N), according to the protein SMAD3. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |