Anti-AKT1 (Ab-450) antibody produced in rabbit
SIGMA/SAB4300575 - affinity isolated antibody
Synonym: Anti-AKT antibody produced in rabbit; Anti-MGC99656 antibody produced in rabbit; Anti-PKB antibody produced in rabbit; Anti-PKB-ALPHA antibody produced in rabbit; Anti-v-akt murine thymoma viral oncogene homolog 1 antibody produced in rabbit
MDL Number: MFCD03096597
Product Type: Chemical
Immunohistochemistry Anti-AKT1 (Ab-450): SAB4300575: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-AKT1 (Ab-450) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-AKT1 (Ab-450): SAB4300575: Staining of methanol-fixed HeLa cells using Anti-AKT1 (Ab-450) antibody (Red).
Immunoblotting Anti-AKT1 (Ab-450): SAB4300575: Analysis of extracts from 293 and MCF cells using Anti-AKT1 (Ab-450) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (T-I-T-P-P) |
| isotype | IgG |
| mol wt | ~60 kDa |
| NCBI accession no. | NP_001014431.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P31749 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. |
| Immunogen: | Peptide sequence around aa. 448-452 (T-I-T-P-P), according to the protein AKT1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |