Anti-CCNB1 (Ab-147) antibody produced in rabbit
SIGMA/SAB4300594 - affinity isolated antibody
Synonym: Anti-CCNB antibody produced in rabbit; Anti-cyclin B1 antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Anti-CCNB1 (Ab-147): SAB4300594: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-CCNB1 (Ab-147) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence Anti-CCNB1 (Ab-147): SAB4300594: Staining of methanol-fixed HeLa cells using Anti-CCNB1 (Ab-147) antibody (Red).
Immunoblotting Anti-CCNB1 (Ab-147): SAB4300594: Analysis of extracts from HepG2 and HT29 cells using Anti-CCNB1 (Ab-147) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (A-F-S-D-V) |
| isotype | IgG |
| mol wt | ~60 kDa |
| NCBI accession no. | NP_114172.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 |
| indirect immunofluorescence: 1:100-1:200 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P14635 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. |
| Immunogen: | Peptide sequence around aa. 145-149 (A-F-S-D-V), according to the protein CCNB1. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |