Anti-phospho-Cdc25b (pSer149) antibody produced in rabbit
SIGMA/SAB4300617 - affinity isolated antibody
Synonym: Anti-AI604853 antibody produced in rabbit; Anti-Cdc25b antibody produced in rabbit; Anti-cell division cycle 25 homolog B (S. pombe) antibody produced in rabbit
MDL Number: MFCD01633249
Product Type: Chemical
Immunoblotting Anti-phospho-Cdc25b (Phospho-Ser149): SAB4300617: Analysis of extracts from 3T3 cells untreated (Lane 1) or treated with Hydroxyurea (Lane 2) using Anti-phospho-Cdc25b (Phospho-Ser149) antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (F-R-SP-L-P) |
| isotype | IgG |
| mol wt | ~62 kDa |
| NCBI accession no. | NP_001104545.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer149) |
| technique(s) | western blot: 1:500-1:1000 |
| UniProt accession no. | P30306 |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. |
| Immunogen: | Peptide sequence around phosphorylation site of Serine 149 (F-R-S(p)-L-P).according to the protein Cdc25b. |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |