Anti-phospho-CK2α (pThr360/Ser362) antibody produced in rabbit
SIGMA/SAB4300628 - affinity isolated antibody
Synonym: Anti-CK2A1; Anti-CKII; Anti-CSNK2A1
Product Type: Chemical
Immunoblotting Anti-phospho-CK2α (pThr360/Ser362) Cat. No. SAB4300628: Analysis of CK2α(pThr360/Ser362) antibody (#SAB4300628) and CK2α antibody (#SAB4300629) in vitro kinase assay. Both purified ERK2 and CK2 were used. CK2α (Phospho-Thr360/Ser362) antibody could recognize ERK2 phosphorylated wild type CK2α, and CK2α when Ser362 was mutated to alanine.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (V-P-Tp-P-Sp-P-L) |
| isotype | IgG |
| mol wt | ~42 kDa |
| NCBI accession no. | NM_001903.2   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr360/pSer362) |
| technique(s) | western blot: 1:500-1:1000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha′ chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates ′Ser-392′ of p53/TP53 following UV irradiation. |
| General description: | The antibody detects endogenous level of CK2 only when phosphorylated at thronine 360. |
| Immunogen: | Peptide sequence around phosphorylation site of threonine360/serine 362 (V-P-Tp-P-Sp-P-L), according to the protein NP_001894.2 |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |