Anti-RUNX1 antibody produced in rabbit
SIGMA/SAB4300709 - affinity isolated antibody
Synonym: Anti-AML1; Anti-AMLCR1; Anti-CBFA2; Anti-EVI-1
Product Type: Chemical
Immunoblotting Anti-RUNX1 Cat. No. SAB4300709: Analysis of extract from P19, HeLa and 293 cells using Anti-RUNX1 antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | (D-A-S-T-S) |
| isotype | IgG |
| mol wt | ~55 kDa |
| NCBI accession no. | NM_001122607.1   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:1000 |
| UniProt accession no. | Q01196 |
| Application: | Anti-RUNX1 antibody produced in rabbit is suitable for western blot at a working dilution of 1:500-1:1000. |
| Biochem/physiol Actions: | RUNX1 (Runt-related transcription factor 1) plays a vital role in tumour suppression and oncogenic activities. In hematopoiesis, it is involed in hematopoietic development, hematopoietic stem cell homeostasis, and various blood malignancies. It may have clinicopathological impact on the proliferation of human bone marrow cells used in transplantation therapy. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | CBF binds to the core site, 5′-PYGPYGGT-3′, of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation. |
| General description: | Runt-related transcription factor 1 (RUNX1) is a transcription factor that plays a vitral role in hematopoiesis, osteogenesis and neurogenesis. It is a member of Runt-related transcription factors (RUNXs). It was first identified as a component of Polyomavirus enhancer binding protein 2 (PEBP2) and Moloney murine leukemia virus enhancer core binding factor (CBF). Three isoforms of the protein have been identified: RUNX1a, RUNX1b and RUNX1c. |
| General description: | The antibody detects endogenous level of total AML1(RUNX1) protein. |
| Immunogen: | Peptide sequence around aa. 6-10 (D-A-S-T-S), according to the protein NP_001116079.1 |
| Physical form: | Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |