Anti-Collagen IV antibody produced in rabbit
SIGMA/SAB4300738 - affinity isolated antibody
Synonym: ALPHA-1 CHAIN ARRESTEN; COL4A1 NC1 domain; COLLAGEN OF BASEMENT MEMBRANE; alpha 1 type IV collagen preproprotein; collagen IV alpha-1 polypeptide
Product Type: Product-on-demand
Immunohistochemistry Anti-Collagen IV : Cat. No. SAB4300738: Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using Anti-Collagen IV antibody. The picture on the right is treated with the synthesized peptide.
Immunoblotting Anti-Collagen IV : Cat. No. SAB4300738: Western blot analysis of extracts from HeLa cells using Anti-Collagen IV antibody. The lane on the right is treated with the synthesized peptide.
| accession no. | NP_001836.2 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG |
| mol wt | 160 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| western blot: 1:500-1:3000 (Cell Lysate) | |
| UniProt accession no. | P02462  |
| Application: | Collagen Type IV from human cell culture has been used in immunofluorescent analysis. |
| Biochem/physiol Actions: | COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication. Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke. |
| Biochem/physiol Actions: | The antibody detects endogenous levels of total Collagen IV protein. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | COL4A1 (collagen type IV α 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats. It is an important constituent of type IV collagen in the basement membrane. The COL4A1 gene is located on human chromosome 13q34. |
| Immunogen: | Synthesized peptide derived from human Collagen IV. |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |