Anti-RAS antibody produced in rabbit
SIGMA/SAB4301113 - affinity isolated antibody
Synonym: CTLO; HAMSV; HRAS1; K-RAS; v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Product Type: Product-on-demand
Immunoblotting Anti-RAS: Cat. No. SAB4301113: Western blot analysis of extracts from HeLa, HepG2 and 231 cells (from left to right) using Anti-RAS antibody.
| accession no. | NP_001123914.1 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.7 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG |
| mol wt | 21 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:200-1:1000 (Cell Lysate) |
| UniProt accession no. | P01112  |
| Biochem/physiol Actions: | Ras is a guanine-nucleotide binding protein that couples tyrosine kinase receptor signals to mitogen activated protein (MAP) kinase cascade. It has an important role in cell growth. Ras is a substrate for farnesyltransferase (FTase) and when farnesylated, it is anchored to the membrane. Membrane localization of Ras is crucial for its cellular activity. Inhibition of Ras farnesylation is a target for anti-cancer drug development. |
| Biochem/physiol Actions: | The antibody detects endogenous level of total RAS protein. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Ras is a proto-oncogene and the gene encoding it is localized on human chromosome 11p15.5. It is a 21 kDa protein and is part of the small GTP-binding proteins superfamily. |
| Immunogen: | Synthetic peptide corresponding to a region derived from 60-74 amino acids of Human v-Ha-ras Harvey rat sarcoma viral oncogene homolog |
| Physical form: | Supplied at 0.7mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |