Anti-6-His antibody produced in rabbit
SIGMA/SAB4301134 - affinity isolated antibody
Synonym: 6 His epitope tag; Anti His Antibody Sigma; Anti His Antibody Sigma - Anti-6×His antibody produced in rabbit
Product Type: Product-on-demand
Immunoblotting Anti-6 ×HIS: Cat. No. SAB4301134: Western blot analysis of Recombinant 6 ×HIS Tagged protein using Anti-6 ×His antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG |
| mol wt | 46 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:2000 (Cell Lysate) |
| Application: | Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells. |
| Application: | The Anti-6-His antibody has been used in western blotting. |
| Biochem/physiol Actions: | The antibody detects transfected proteins contanining 6×His tag. |
| Biochem/physiol Actions: | The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site. |
| Immunogen: | Synthetic peptide: 18XHis conjugated to KLH |
| Physical form: | Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |