Anti-FLAG Tag antibody produced in rabbit
SIGMA/SAB4301135 - affinity isolated antibody
Synonym: Anti-ddddk; Anti-dykddddk
Product Type: Product-on-demand
Immunoblotting Anti-FLAG Tag: Cat. No. SAB4301135: Western blot analysis of extracts from 293T cells using Anti-FLAG Tag antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.4 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| immunogen sequence | DYKDDDDK conjugated to KLH (synthetic peptide) |
| isotype | IgG |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | western blot: 1:1000-1:5000 (Cell Lysate) |
| Application: | Anti-FLAG Tag antibody produced in rabbit has been used in: • western blotting • co-immunoprecipitation • immunofluorescence studies |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Learn more product details in our FLAG® application portal. |
| Biochem/physiol Actions: | The antibody detects transfected proteins contanining FLAG tag. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The FLAG epitope is composed of eight amino acids with the following sequence AspTyrLysAspAspAspAspLys. It helps in immunoaffinity purification of fusion proteins. |
| Immunogen: | Synthetic peptide: DYKDDDDK conjugated to KLH |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |