Anti-GFP antibody produced in rabbit
SIGMA/SAB4301138 - affinity isolated antibody
Synonym: GFP-like chromoprotein
Product Type: Product-on-demand
Immunoblotting Anti-GFP: Cat. No. SAB4301138: Western blot analysis of extracts from GFP transfected 293T cells (1ug-20ug) using Anti-GFP antibody.
Immunoblotting Anti-GFP: Cat. No. SAB4301138: Western blot analysis of extracts from GFP transfected 293T cells (1ug-20ug) using Anti-GFP antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 3.2 mg/mL |
| form | buffered aqueous solution |
| isotype | IgG |
| mol wt | 27 kDa |
| Quality Level | 100  |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:1000-1:10000 (Cell Lysate) |
| Application: | Anti-GFP antibody produced in rabbit has been used in: • double immunofluorescence staining • immunoblot analysis. • immunoprecipitation. |
| Biochem/physiol Actions: | Green fluorescent protein (GFP) is a reporter molecule which is used for checking gene expression and protein localization in vivo, in situ and in real time. GFP emits green light when it is excited with UV/blue light. The GFP fluorescence remains stable and can be detected non-invasively in living cells. GFP is considered as a unique tool to monitor dynamic processes in several living cells or organisms. When expressed in either eukaryotic or prokaryotic cells and illuminated by blue or UV light, GFP yields a bright green fluorescence. |
| Biochem/physiol Actions: | The antibody detects transfected proteins containing GFP tag. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Green fluorescent protein (GFP) is a 27kDa protein, derived from the bioluminescent jellyfish Aequorea victoria, in which light is produced when energy is transferred from the Ca2+- activated photoprotein aequorin to GFP. |
| Immunogen: | Full length fusion protein |
| Physical form: | Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |