Anti-CD274
SIGMA/SAB4301882 - affinity isolated antibody
Synonym: B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1
Product Type: Product-on-demand
Immunohistochemistry Anti-CD274: Cat. No. SAB4301882: The image on the left is immunohistochemistry of paraffin-embedded Human Esophagus cancer tissue at dilution 1/30, on the right is treated with synthetic peptide.
Immunoblotting Anti-CD274: Cat. No. SAB4301882: Gel: 8% SDS-PAGE Lysate: 40 μg. Lane 1-5: A549, Raji, RAW264.7, Jurkat and HEPG2 cell lysates. Primary antibody: CD274 antibody at dilution 1/250. Secondary antibody: Goat Anti-Rabbit IgG at 1/8,000 dilution. Exposure time: 20 seconds.
| accession no. | NP_054862 |
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1: 20-100 |
| western blot: 1:200-1:1000 | |
| UniProt accession no. | Q9NZQ7  |
| Biochem/physiol Actions: | Programmed cell death 1 ligand 1 (PD-L1) is involved in the regulation of cellular and humoral immune responses. (4) PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after lipopolysaccharide (LPS) and interferon-γ (IFNG) activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 is involved in the co-stimulatory signal, essential for T-cell proliferation and production of interleukin-10 (IL10) and IFNG, in an interleukin-2-dependent and a programmed cell death 1 (PDCD1)-independent manner. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Programmed cell death 1 ligand 1 (PD-L1), which is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules. PD-L1 is a cell surface immunoglobulin with two Ig-like domains within the extracellular region and a short cytoplasmic domain. The protein is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. The gene encoding it is localized on human chromosome 9p24.1. |
| Immunogen: | Synthetic peptide of human CD274 |
| Physical form: | Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |