Anti-BCL-2 antibody produced in rabbit
SIGMA/SAB4500003 - affinity isolated antibody
Synonym: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; BCL2
MDL Number: MFCD00239644
Product Type: Chemical
Immunohistochemistry Anti-BCL-2: Cat. No. SAB4500003: Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-BCL-2 antibody. Cells on the left were treated with the Anti-BCL-2 antibody. Cells on the right (negative control) were treated with both Anti-BCL-2 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-BCL-2: Cat. No. SAB4500003: Immunofluorescence of HepG2 cells using Anti-BCL-2 antibody. Cells on the left were treated with the Anti-BCL-2 antibody. Cells on the right (negative control) were treated with both Anti-BCL-2 antibody and the synthesized immunogen peptide.
Western Blotting Anti-BCL-2: Cat. No. SAB4500003: Immunoblotting analysis of extracts from K562 cells, using Anti-BCL-2 antibody. The lane on the left was treated with the Anti-BCL-2 antibody. The lane on the right (negative control) was treated with both Anti-BCL-2 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 26 kDa |
| NCBI accession no. | P10415   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P10415 |
| Application: | Anti-BCL-2 antibody produced in rabbit has been used in western blotting. |
| Biochem/physiol Actions: | BCL2 is an apoptosis regulator that is expressed in sub-cellular components such as the mitochondria and nucleus. While mitochondrial BCL2 inhibits apoptosis, BCL2 present in the nucleus may activate cell death. BCL2 may also regulate autophagy during starvation . Anti-BCL-2 antibodies are specific for endogenous levels of total BCL-2 protein in rats, humans and mice. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | B-cell lymphoma 2 (BCL-2) is an integral inner mitochondrial membrane protein that acts as an antiapoptotic protein. It is a target gene of nuclear factor kappa B (NF-kB). The gene encoding BCL-2 is localized on human chromosome 18q21. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human BCL-2. Immunogen Range: 46-95 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |