Anti-HDAC6 antibody produced in rabbit
SIGMA/SAB4500011 - affinity isolated antibody
Synonym: HD6; HDA6; Histone deacetylase 6; JM21
Product Type: Chemical
Immunohistochemistry Anti-HDAC6: Cat. No. SAB4500011: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-HDAC6 antibody. Cells on the left were treated with the Anti-HDAC6 antibody. Cells on the right (negative control) were treated with both Anti-HDAC6 antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-HDAC6: Cat. No. SAB4500011: Immunofluorescence of HepG2 cells using Anti-HDAC6 antibody. Cells on the left were treated with the Anti-HDAC6 antibody. Cells on the right (negative control) were treated with both Anti-HDAC6 antibody and the synthesized immunogen peptide.
Western Blotting Anti-HDAC6: Cat. No. SAB4500011: Immunoblotting analysis of extracts from HepG2 cells, using Anti-HDAC6 antibody. The lane on the left was treated with the Anti-HDAC6 antibody. The lane on the right (negative control) was treated with both Anti-HDAC6 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 131 kDa |
| NCBI accession no. | Q9UBN7   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9UBN7 |
| Biochem/physiol Actions: | Histone deacetylase 6 (HDAC6) deacetylates substrates like α-tubulin. It is involved in endocytosis and autophagy. HDAC6 functions in cellular mechanisms connected to cancer and modulates cell motility. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Histone deacetylase 6 (HDAC6) is a class IIb histone deacetylase which is localized in the cytosol. It has two catalytic domains. The protein is expressed in the heart, brain, colon, breast, ovary, pancreas and prostate glands. The gene encoding HDAC6 is localized on human chromosome Xp11.23. Anti-HDAC6 Antibody detects endogenous levels of total HDAC6 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human HDAC6. Immunogen Range: 7-56 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |