Anti-AOS1 antibody produced in rabbit
SIGMA/SAB4500028 - affinity isolated antibody
Synonym: AOS1; SAE1; SUA1; SUMO-activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A
MDL Number: MFCD03454733
Product Type: Chemical
Immunofluorescence Anti-AOS1: Cat. No. SAB4500028: Immunofluorescence of HuvEc cells using Anti-AOS1 antibody. Cells on the left were treated with the Anti-AOS1 antibody. Cells on the right (negative control) were treated with both Anti-AOS1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-AOS1: Cat. No. SAB4500028: Immunoblotting analysis of extracts from 293 cells, using Anti-AOS1 antibody. The lane on the left was treated with the Anti-AOS1 antibody. The lane on the right (negative control) was treated with both Anti-AOS1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 38 kDa |
| NCBI accession no. | Q9UBE0   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9UBE0 |
| Biochem/physiol Actions: | SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1)/ AOS1 transcription is induced by Myc via direct binding to canonical E-Boxes sequences present near the SAE1 transcription start site. SAE1 and SAE2/ UBA2 complex plays a crucial role in the tumorigenicity of Myc-dependent cancer cells in vitro and in vivo. SAE1/SAE2 heterodimer complex catalyzes the conjugation of SUMO-1 to a variety of cellular substrates via ATP-dependent reaction, where the first step involves C-terminal adenylation of glycine residues of SUMO-1 and second step involves thioester bond formation between C-terminal glycine of SUMO-1 and a cysteine residue in SAE2. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | E1 ubiquitin-activating protein AOS1 also known as SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1) is encoded by the gene mapped to human chromosome 19. Human ASO1 is expressed throughout nuclei apart from nucleoli.The protein expression is elevated in S phase and decreased in G2 phase of cell cycle. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human AOS1. Immunogen Range: 221-270 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |