Anti-p27 KIP1 antibody produced in rabbit
SIGMA/SAB4500068 - affinity isolated antibody
Synonym: CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1
Product Type: Chemical
Immunohistochemistry Anti-p27 KIP1: Cat. No. SAB4500068: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-p27 KIP1 antibody. Cells on the left were treated with the Anti-p27 KIP1 antibody. Cells on the right (negative control) were treated with both Anti-p27 KIP1 antibody and the synthesized immunogen peptide.
Western Blotting Anti-p27 KIP1: Cat. No. SAB4500068: Immunoblotting analysis of extracts from A2780 cells, using Anti-p27 KIP1 antibody. The lane on the left was treated with the Anti-p27 KIP1 antibody. The lane on the right (negative control) was treated with both Anti-p27 KIP1 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 22 kDa |
| NCBI accession no. | P46527   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P46527 |
| Application: | Anti-p27 KIP1 antibody produced in rabbit has been used in western blotting (1:500). |
| Biochem/physiol Actions: | Cyclin-dependent kinase inhibitor 1B (CDKN1B) negatively regulates cell cycle events by binding to cyclin-dependent kinase. It is a tumor suppressor gene correlated with the pathophysiology of tumors. Germline mutations in CDKN1B are implicated in multiple endocrine neoplasia (MEN) syndrome and MEN-like syndrome (MENX). It is a potent biomarker in acute myeloid leukemia. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-p27 KIP1 Antibody detects endogenous levels of total p27 KIP1 protein. |
| General description: | Cyclin-dependent kinase inhibitor 1B (CDKN1B or p27 KIP1) is a member of CDK inhibitor (CKI). The CDKN1B gene is mapped to human chromosome 12p13.1. The prime domains in CDKN1B include the nuclear export signal (NES) and the nuclear localization signal (NLS) and the CDK binding domain. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p27 Kip1. Immunogen Range: 1-50 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |