Anti-p57 KIP2 antibody produced in rabbit
SIGMA/SAB4500071 - affinity isolated antibody
Synonym: CDKN1C; CDN1C; CDNC; Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57
Product Type: Chemical
Immunofluorescence Anti-p57 KIP2: Cat. No. SAB4500071: Immunofluorescence of HuvEc cells using Anti-p57 KIP2 antibody. Cells on the left were treated with the Anti-p57 KIP2 antibody. Cells on the right (negative control) were treated with both Anti-p57 KIP2 antibody and the synthesized immunogen peptide.
Western Blotting Anti-p57 KIP2: Cat. No. SAB4500071: Immunoblotting analysis of extracts from K562 cells, using Anti-p57 KIP2 antibody. The lane on the left was treated with the Anti-p57 KIP2 antibody. The lane on the right (negative control) was treated with both Anti-p57 KIP2 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 32 kDa |
| NCBI accession no. | P49918   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P49918 |
| Application: | Anti-p57 KIP2, C-Terminal antibody produced in rabbit has been used for Western blot analysis. |
| Biochem/physiol Actions: | Cyclin-dependent kinase inhibitor p57 (CDKN1C) or p57 KIP2, plays a vital role as a tight-binding inhibitor of several G1 cyclin/ cyclin-dependent kinase (CDK) complexes. The encoded protein acts as a negative regulator of cell proliferation. Mutation of this gene results in Beckwith-Wiedemann syndrome. p57Kip2 protein is involved in the glucocorticoid mediated inhibition of cell proliferation in HeLa cells. p57Kip2 also facilitates direct inhibition of DNA replication by binding to the proliferating cell nuclear antigen. p57Kip2 is a tumor suppressor protein, and helps in normal developmental process. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Cyclin-dependent kinase inhibitor p57 (CDKN1C) or p57 KIP2 (Kinase inhibitory protein) is a tumor suppressor gene mapped to telomeric end of human chromosome 11p15.5, a region associated with sporadic cancers and Beckwith-Wiedemann syndrome. Human p57KIP2 codes for a 316 amino acid protein characterized with conserved amino- and carboxy-terminal domains, and consisting of sequences with proline-alanine repeats. During mouse embryogenesis, p57 KIP2 transcript is expressed highly in skeletal muscle, brain, heart, lungs, and eye. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p57 Kip2. Immunogen Range: 267-316 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |