Anti-p16 INK antibody produced in rabbit
SIGMA/SAB4500072 - affinity isolated antibody
Synonym: CD2A1; CDK4I; CDKN2; CDKN2A; CDN2
Product Type: Chemical
Western Blotting Anti-p16 INK: Cat. No. SAB4500072: Immunoblotting analysis of extracts from HeLa cells, using Anti-p16 INK antibody. The lane on the left was treated with the Anti-p16 INK antibody. The lane on the right (negative control) was treated with both Anti-p16 INK antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 16 kDa |
| NCBI accession no. | P42771   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P42771 |
| Application: | Anti-p16 INK, N-Terminal antibody produced in rabbit has been used in western blot analysis. |
| Biochem/physiol Actions: | Cyclin dependent kinase inhibitor 2A (CDKN2A) also known as P16-INK4A, arrests cell cycle progression by inhibiting S-phase and promotes apoptosis. The encoded protein has anti-proliferative biological activity, and is implicated in the retinoblastoma (Rb) protein pathway. P16-INK4A acts as a tumor suppressor and plays a vital role in ageing and cellular senescence. Increased expression of this protein might lead to various types of high grade cancers. P16-INK4A also facilitates cell invasion and angiogenesis. Bronchial lavage P16INK4A promoter methylation might function as a potential biomarker for diagnosis of lung cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-p16 INK Antibody detects endogenous levels of total p16 INK protein. |
| General description: | Cyclin dependent kinase inhibitor 2A (CDKN2A) also known as P16-INK4A, belongs to the Ink4 (inhibitor of CDK4) family of CDK inhibitors. It is encoded by the gene mapped to human chromosome 9p21 within the INK4a / alternate reading frame (ARF) locus. Human P16-INK4A is localized in both nucleus and cytoplasm, and its expression increases with ageing in skin and kidney tissues. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human p16 INK. Immunogen Range: 10-59 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |