Anti-Tubulin β antibody produced in rabbit
SIGMA/SAB4500088 - affinity isolated antibody
Synonym: TUBB3; TUBB4; Tubulin β-3 chain; Tubulin β-4; Tubulin β-III
MDL Number: MFCD01322746
Product Type: Chemical
Immunohistochemistry Anti-Tubulin β: Cat. No. SAB4500088: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Tubulin β antibody. Cells on the left were treated with the Anti-Tubulin β antibody. Cells on the right (negative control) were treated with both Anti-Tubulin β antibody and the synthesized immunogen peptide.
Western Blotting Anti-Tubulin β: Cat. No. SAB4500088: Immunoblotting analysis of extracts from mouse brain/rat brain cells, using Anti-Tubulin β antibody. The lane on the left was treated with the Anti-Tubulin β antibody. The lane on the right (negative control) was treated with both Anti-Tubulin β antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 50 kDa |
| NCBI accession no. | Q13509   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q13509 |
| Application: | Anti-Tubulin β C-Terminal antibody has been used in immunoblot assays. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) |
| Biochem/physiol Actions: | Upregulation of tubulin β-III (TUBB3) shows vigorous tumor characteristics and genetic instability in urinary bladder cancer. It participates in the separation of chromosome. Individuals with congenital fibrosis of the extraocular muscle type 3 (CFEOM3) show mutations in TUBB3. βIII-tubulin modulates glucose metabolism, thereby transforms glucose starvation stress signaling to develop the multiplication of cell and survival in non-small cell lung cancer cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Tubulin β-III (TUBB3) is a constituent of microtubules. It is expressed in mature neurons. It is located on human chromosome 16q24. Anti-Tubulin |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Tubulin beta. Immunogen Range: 401-450 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |