Anti-YAP antibody produced in rabbit
SIGMA/SAB4500094 - affinity isolated antibody
Synonym: 65 kDa Yes-associated protein; YAP1; YAP65
MDL Number: MFCD04118707
Product Type: Chemical
Western Blotting Anti-YAP: Cat. No. SAB4500094: Immunoblotting analysis of extracts from HepG2 cells, treated with Wortmannin 40nM 24h, using Anti-YAP antibody. The lane on the left was treated with the Anti-YAP antibody. The lane on the right (negative control) was treated with both Anti-YAP antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 48 kDa |
| NCBI accession no. | P46937   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P46937 |
| Biochem/physiol Actions: | Yes associated protein (YAP) serves as a potent mechanosensitive transcriptional activator. It plays a vital role in regulation of cancer, regeneration and organ size. Overexpression of the gene contributes to tumorigenesis in breast and liver cancers. In addition, it also promotes gallbladder cancer (GBC) cell growth via activating AXL receptor tyrosine kinase/mitogen-activated protein kinase (MAPK) pathway. Thus, it acts as a potent biomarker and therapeutic target for various cancers including, GBC and breast cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-YAP Antibody detects endogenous levels of total YAP protein. |
| General description: | Yes associated protein (YAP) is a 65kDa proline-rich phosphoprotein. It is encoded by the gene mapped to human chromosome 11q22.1. The encoded protein is characterized with a WW domain, a PDZ interaction motif, an SH3 binding motif and a coiled-coil domain. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human YAP. Immunogen Range: 93-142 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |