Anti-C/EBP-β antibody produced in rabbit
SIGMA/SAB4500112 - affinity isolated antibody
Synonym: AGP/EBP; C/EBP β; C/EBP-related protein 2; CCAAT/enhancer binding protein β; CEBPB
MDL Number: MFCD04118430
Product Type: Chemical
Immunohistochemistry JOURNAL CITATION: C/EBPβ: enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. By: Liu, D., Zhang, X. X., et al. in Nat Commun, 2018. PubMed ID: 29712898 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41467-018-03590-5), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: C/EBPβ: enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. By: Liu, D., Zhang, X. X., et al. in Nat Commun, 2018. PubMed ID: 29712898 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41467-018-03590-5), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: C/EBPβ: enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. By: Liu, D., Zhang, X. X., et al. in Nat Commun, 2018. PubMed ID: 29712898 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/articles/s41467-018-03590-5), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Anti-C/EBP-β: Cat. No. SAB4500112: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-C/EBP-β antibody. Cells on the left were treated with the Anti-C/EBP-β antibody. Cells on the right (negative control) were treated with both Anti-C/EBP-β antibody and the synthesized immunogen peptide.
Western Blotting Anti-C/EBP-β: Cat. No. SAB4500112: Immunoblotting analysis of extracts from NIH-3T3 cells, using Anti-C/EBP-β antibody. The lane on the left was treated with the Anti-C/EBP-β antibody. The lane on the right (negative control) was treated with both Anti-C/EBP-β antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 36 kDa |
| NCBI accession no. | P17676   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P17676 |
| Biochem/physiol Actions: | CCAAT/enhancer-binding proteins (C/EBPs) regulate expression of various cytokines and chemokines such as, interleukin-6 (IL-6), IL-8 and monocyte chemoattractant protein-1 (MCP-1). C/EBP-β plays a vital role in the regulation of gluconeogenesis and adipogenesis gene expression. The encoded protein also modulates the gene expression of human immunodeficiency virus-1 (HIV-1) and other retroviruses (human T-cell leukemia virus type (HTLV-) and simian immunodeficiency viruses (SIV)). This is carried out by regulating various process, like C-C chemokine receptor type 5 (CCR5) expression, virus promoter activities, cytokine and chemokine production and virus protein function. C/EBPβ is involved in various cellular processes such as metabolism, development and differentiation. Overexpression of C/EBP-β mRNA has been observed in various types of cancers. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-C/EBP-β Antibody detects endogenous levels of total C/EBP-β protein. |
| General description: | CCAAT/enhancer-binding protein-β (C/EBP-β) is encoded by the gene mapped to human chromosome 20q13.13. The encoded protein belongs to the C/EBP family. C/EBP-β is a leucine-zipper transcription factor and is highly expressed during the early stages of adipocyte development. Alternative splicing of C/EBP-β leads to the formation of three isoforms such as, C/EBPβ-1 with 346 amino acids, C/EBPβ-2 with 323 amino acid and C/EBPβ with 146 amino acids. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human C/EBP-beta. Immunogen Range: 201-250 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |