Anti-PLK2 antibody produced in rabbit
SIGMA/SAB4500156 - affinity isolated antibody
Synonym: PLK-2; Polo-like kinase 1; Serine/
Product Type: Chemical
Western Blotting Anti-PLK2: Cat. No. SAB4500156: Immunoblotting analysis of extracts from HeLa/MCF-7 cells, using Anti-PLK2 antibody. The lane on the left was treated with the Anti-PLK2 antibody. The lane on the right (negative control) was treated with both Anti-PLK2 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 78 kDa |
| NCBI accession no. | Q9NYY3   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:40000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | Q9NYY3 |
| Application: | Anti-PLK2 antibody produced in rabbit has been used in western blotting(1:1000) (1:5000) |
| Biochem/physiol Actions: | Polo-like kinase 2 (PLK2) participates in regulating centriole duplication, cell death, and cell cycle. It also mediates the spine-associated RapGAP (SPAR) degradation via the ubiquitin-proteasome pathway, thereby regulating synaptic plasticity. Mutations in the PLK2 gene are observed in colorectal cancers. The PLK2 gene knockdown leads to defects in centriole duplication. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-PLK2 Antibody detects endogenous levels of total PLK2 protein. |
| General description: | Polo-like kinases 2 (PLK2) is a member of the serine-threonine kinases family. The PLK2 gene is mapped to human chromosome 5q11.2. PLK2 comprises a C-terminal polo-box domain (PBD) devoid of catalytic activity and an N-terminal kinase domain. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PLK2. Immunogen Range: 291-340 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |