Anti-RPL35 antibody produced in rabbit
SIGMA/SAB4500233 - affinity isolated antibody
Synonym: 60S ribosomal protein L35
Product Type: Chemical
Western Blotting Anti-RPL35: Cat. No. SAB4500233: Immunoblotting analysis of extracts from 293/HeLa/COLO/Jurkat cells, using Anti-RPL35 antibody. The lane on the left was treated with the Anti-RPL35 antibody. The lane on the right (negative control) was treated with both Anti-RPL35 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 14 kDa |
| NCBI accession no. | P42766   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:10000 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P42766 |
| Application: | Anti-RPL35 antibody produced in rabbit has been used in immunofluorescence. |
| Biochem/physiol Actions: | Ribosomal protein L35 (RPL35) protein modulates methionine (Met)-mediated β-casein (CSN2) translational elongation and secretion. Lack of RPL35A can enhance apoptosis in hematopoietic cell lines and bone marrow cells. RPL35 gene is regarded as one of the most reliable reference genes to study gene expression in developing cacao embryos. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Ribosomal protein L35 (RPL35) gene codes for a ribosomal protein, a constituent of the 60S subunit. It is located on human chromosome 9q33.3. Anti-RPL35 antibody detects endogenous levels of total RPL35 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human RPL35. Immunogen Range: 51-100 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |