Anti-Histone H3 antibody produced in rabbit
SIGMA/SAB4500352 - affinity isolated antibody
Synonym: H3/a; H3/c; H3/d; H3/f; H3/h
Product Type: Chemical
Immunohistochemistry Anti-Histone H3: Cat. No. SAB4500352: Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Anti-Histone H3 antibody. Cells on the left were treated with the Anti-Histone H3 antibody. Cells on the right (negative control) were treated with both Anti-Histone H3 antibody and the synthesized immunogen peptide.
Western Blotting Anti-Histone H3: Cat. No. SAB4500352: Immunoblotting analysis of extracts from Raw264.7 cells, treated with TSA 400nM 24h, using Anti-Histone H3 antibody. The lane on the left was treated with the Anti-Histone H3 antibody. The lane on the right (negative control) was treated with both Anti-Histone H3 antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 15 kDa |
| NCBI accession no. | P68431   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 |
| Application: | Anti-Histone H3, N-Terminal antibody has been used in western blotting and enzyme-linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | Histone proteins are basic building blocks of chromatin. Histone H3 K27M mutations in adult cerebellar high-grade gliomas. It controls protein-protein interactions to induce binding of trans-acting factors that “drive” chromatin condensation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Histone H3 Antibody detects endogenous levels of total Histone H3 protein. |
| General description: | Mammals contain three main classes of histone H3 variants: the replicative histones (H3.1 and H3.2), the replacement histone (H3.3) and the centromeric histone (Cenp-A). Histone H3 is located on human chromosome 6p22. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Histone H3. Immunogen Range: 1-50 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |