Anti-Collagen I antibody produced in rabbit
SIGMA/SAB4500362 - affinity isolated antibody
Synonym: α 2 type i collagen; co1a2; col1a2; collagen; type i
Product Type: Chemical
Immunohistochemistry Anti-Collagen I: Cat. No. SAB4500362: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Collagen I antibody. Cells on the left were treated with the Anti-Collagen I antibody. Cells on the right (negative control) were treated with both Anti-Collagen I antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Collagen I: Cat. No. SAB4500362: Immunofluorescence of NIH-3T3 cells using Anti-Collagen I antibody. Cells on the left were treated with the Anti-Collagen I antibody. Cells on the right (negative control) were treated with both Anti-Collagen I antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 129 kDa |
| NCBI accession no. | P08123   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| UniProt accession no. | P08123 |
| Application: | Anti-Collagen I antibody produced in rabbit has been used in western blotting and immunostaining. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Application: | Rabbit polyclonal anti-Collagen I, N-Terminal antibody is used to tag collagen type I for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It is used as a probe to determine the presence and roles of collagen type I as a cell substrate. |
| Biochem/physiol Actions: | Collagen I interacts with cytoskeleton and regulates cell motility. Mutations in this gene is linked to osteoporotic fracture, osteogenesis imperfecta and recessive classical type Ehlers-Danlos syndrome. It is also associated with idiopathic osteoporosis and atypical Marfan syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Collagen I is encoded by the gene COL1A2 (collagen type I alpha 2). The gene is located on human chromosome 7q21.3. COL1A2 is a fibril forming collagen, found in bone, dermis, tendon, ligaments and cornea. |
| General description: | Collagen is the major structural component of extracellular matrices found in connective tissues and internal organs, but is most prevalent in the dermis, tendons, and bones. It is classified into a number of structurally and genetically distinct types. Type I collagen is a heterodimer composed of two α1(I) chains and one α2(I) chain that spontaneously forms a triple helix scaffold at neutral pH and 37 °C. Collagen type I is an excellent substrate for the culture of hepatocytes, fibroblasts, spinal ganglion, muscle cells, Schwann cells, embryonic lung cells, epithelial cells, and a number of other cell lines. It has also been used in the study of growth, differentiation, migration of cell lines, and tissue morphogenesis during development. |
| General description: | Rabbit polyclonal anti-Collagen I, N-Terminal antibody reacts with mouse, rat, and human type I collagens. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Collagen I. Immunogen Range: 1-50 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |