Anti-Collagen II antibody produced in rabbit
SIGMA/SAB4500366 - affinity isolated antibody
Synonym: CO2A1; chondrocalcin; collagen; collagen α-1 (II); type II
Product Type: Chemical
Immunohistochemistry Anti-Collagen II: Cat. No. SAB4500366: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Collagen II antibody. Cells on the left were treated with the Anti-Collagen II antibody. Cells on the right (negative control) were treated with both Anti-Collagen II antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Collagen II: Cat. No. SAB4500366: Immunofluorescence of COS7 cells using Anti-Collagen II antibody. Cells on the left were treated with the Anti-Collagen II antibody. Cells on the right (negative control) were treated with both Anti-Collagen II antibody and the synthesized immunogen peptide.
Western Blotting Anti-Collagen II: Cat. No. SAB4500366: Immunoblotting analysis of extracts from COLO205 cells, using Anti-Collagen II antibody. The lane on the left was treated with the Anti-Collagen II antibody. The lane on the right (negative control) was treated with both Anti-Collagen II antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 141 kDa |
| NCBI accession no. | P02458   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P02458 |
| Application: | Anti-Collagen II, N-Terminal antibody produced in rabbit has been used for: • Western blotting • Immunofluorescence • Immunohistological analyses |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (2 papers) |
| Biochem/physiol Actions: | Mutation of collagen type II α 1 chain (COL2A1), leads to a variety of clinical manifestations such as skeletal dysplasia, short stature, and sensorial defects, collectively known as type II collagenopathies. Function of COL2A1 in articular cartilage is to form highly organized fibers, which are essential for the mechanical properties of the tissue. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Collagen II Antibody detects endogenous levels of total Collagen II protein. |
| General description: | Collagen type II α 1 chain (COL2A1) gene is mapped to human chromosome 12q13.11–q13.12. COL2A1 belongs to collagen superfamily. The encoded protein is characterized with three identical α chains forming homotrimers. Collagen II is the major component of cartilage. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Collagen II. Immunogen Range: 101-150 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |