Anti-Collagen III antibody produced in rabbit
SIGMA/SAB4500367 - affinity isolated antibody
Synonym: COL3A1; Collagen α-1(III) chain
Product Type: Chemical
Immunohistochemistry Anti-Collagen III: Cat. No. SAB4500367: Immunohistochemistry analysis of paraffin-embedded human pancreas tissue using Anti-Collagen III antibody. Cells on the left were treated with the Anti-Collagen III antibody. Cells on the right (negative control) were treated with both Anti-Collagen III antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-Collagen III: Cat. No. SAB4500367: Immunofluorescence of HeLa cells using Anti-Collagen III antibody. Cells on the left were treated with the Anti-Collagen III antibody. Cells on the right (negative control) were treated with both Anti-Collagen III antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 138 kDa |
| NCBI accession no. | P02461   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:5000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| UniProt accession no. | P02461 |
| Application: | Anti-Collagen III antibody has been used in immunohistochemistry. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | Collagens usually help in the development of fibrillar and microfibrillar networks of the extracellular matrix, basement membranes and structures of the extracellular matrix. Mutations in the COL3A1 gene results in vascular ehlers-danlos syndrome (vEDS). COL3A1 controls the expression and modulation of collagen. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Collagen III Antibody detects endogenous levels of total Collagen III protein. Collagen III is also termed as collagen type III α1 (COL3A1). It is a fibrillar collagen present in extensible connective tissues. COL3A1 spans around 44 kb in length and has 52 exons. It is expressed in blood vessels and hollow organs. Collagen III gene is located on human chromosome 2q32.2. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Collagen III. Immunogen Range: 81-130 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |