Anti-Collagen IV antibody produced in rabbit
SIGMA/SAB4500369 - affinity isolated antibody
Synonym: Arresten; COL4A1; Collagen α-1(IV) chain
Product Type: Chemical
Immunohistochemistry Anti-Collagen IV: Cat. No. SAB4500369: Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-Collagen IV antibody. Cells on the left were treated with the Anti-Collagen IV antibody. Cells on the right (negative control) were treated with both Anti-Collagen IV antibody and the synthesized immunogen peptide.
Western Blotting Anti-Collagen IV: Cat. No. SAB4500369: Immunoblotting analysis of extracts from HeLa cells, using Anti-Collagen IV antibody. The lane on the left was treated with the Anti-Collagen IV antibody. The lane on the right (negative control) was treated with both Anti-Collagen IV antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 160 kDa |
| NCBI accession no. | P02462   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:20000 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P02462 |
| Application: | Anti-Collagen IV, N-Terminal antibody has been used in histology analysis, immunofluorescence microscopy and immunostaining. |
| Biochem/physiol Actions: | COL4A1 (collagen type IV α 1 chain) related disorder is associated with pulmonary complication. This gene is a major constituent required in basal membrane stability. Mutations in COL4A1 result in familial porencephaly, infantile hemiplegia, cerebral small vessel disease (CSVD) and hemorrhagic stroke. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Anti-Collagen IV Antibody detects endogenous levels of total Collagen IV protein. |
| General description: | COL4A1 (collagen type IV alpha 1 chain) belongs to the collagen family. It has an N-terminal 7S domain, a C-terminal globular domain (NC1), and the central triple helical part with short interruptions of the Gly-X-Y repeats. It is an important constituent of type IV collagen in the basement membrane. The COL4A1 gene is located on human chromosome 13q34. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Collagen IV. Immunogen Range: 11-60 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |