Anti-ADORA2A antibody produced in rabbit
SIGMA/SAB4500423 - affinity isolated antibody
Synonym: ADORA2; ADORA2A; Adenosine receptor A2a
Product Type: Chemical
Immunohistochemistry Anti-ADORA2A: Cat. No. SAB4500423: Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-ADORA2A antibody. Cells on the left were treated with the Anti-ADORA2A antibody. Cells on the right (negative control) were treated with both Anti-ADORA2A antibody and the synthesized immunogen peptide.
Immunofluorescence Anti-ADORA2A: Cat. No. SAB4500423: Immunofluorescence of LOVO cells using Anti-ADORA2A antibody. Cells on the left were treated with the Anti-ADORA2A antibody. Cells on the right (negative control) were treated with both Anti-ADORA2A antibody and the synthesized immunogen peptide.
Western Blotting Anti-ADORA2A: Cat. No. SAB4500423: Immunoblotting analysis of extracts from HepG2 cells, using Anti-ADORA2A antibody. The lane on the left was treated with the Anti-ADORA2A antibody. The lane on the right (negative control) was treated with both Anti-ADORA2A antibody and the synthesized immunogen peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 44 kDa |
| NCBI accession no. | P29274   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1000 |
| immunofluorescence: 1:100-1:500 | |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P29274 |
| Biochem/physiol Actions: | Activation of the adenosine A2a receptor (ADORA2A) prevents T-cell proliferation and production of pro-inflammatory cytokines. It functions in increasing cytosolic cAMP levels. The protein has been associated with stimulation of liver ischemic preconditioning. ADORA2A is also linked to dermal fibrosis and scleroderma. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Adenosine A2a receptor (ADORA2A) is a G protein-coupled receptor. It is highly expressed in the brain. It is located on human chromosome 22q11.23. |
| General description: | Anti-ADORA2A Antibody detects endogenous levels of total ADORA2A protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human ADORA2A. Immunogen Range: 321-370 |
| Physical form: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |